Microbiome Related Cytotoxically Active CD8+ TIL Are Inversely Associated With Lung Cancer Development

作者：Leliang Zheng, Jiaqi Xu, Buqing Sai, Yinghong Zhu, Lujuan Wang, Na Yin, Fenglei Yu, Wen Zhou, Minghua Wu, Jingqun Tang, Juanjuan Xiang · 发表于：Frontiers in Oncology · 年份：2020 · DOI：10.3389/fonc.2020.531131 · 被引用次数：28 · 研究领域：Cancer Research and Treatments、Gut microbiota and health、Cancer Cells and Metastasis

Lung cancer is the most common cancer type around the world. Although major advances in cancer therapy, lung cancer has been the largest proportion of all cancer-related deaths. The respiratory tract contains many types of bacteria and a distinct lung microbiome in lung cancer patients was described in many studies. The specific roles of these lung microorganisms in lung cancer progression remain unclear. In this study, we evaluated the effect of inhalation of bronchoalveolar fluid (BAL) in the lung cancer cell growth. The microbiome-based immune and carcinogenesis was examined in tumor-bearing mouse model. We found that inhalation of BAL collected from non-small cell lung cancer (NSCLC) patients altered the lung microbiota and inhibited tumor cell growth. The inhibitory effect was due to the infiltration of CD3 and CD8 + T cells and decrease of M2 macrophages in lungs. The microbial communities of NSCLC BAL inhalation group were dominated by Pasteurella , whereas the microbial communities of non-cancer control and PBS inhalation group were dominated by Delftia . Linear discriminant analysis (LDA) indicated that the genera Pasteurella , Pseudomonas , and Chryseobacterium were increased in NSCLC BAL inhalation group, while genera Delftia , Ezakiella , Blautia , Cloacibacterium , and Microvirga et al. were increased in PBS and Non-cancer group. We demonstrated a significant positive correlation between Pasteurella and cytotoxic CD8 + TIL and a negative correlation with M2 macro...