ProbeDealer is a convenient tool for designing probes for highly multiplexed fluorescence in situ hybridization

作者：Mengwei Hu, Bing Yang, Yubao Cheng, Jonathan S. D. Radda, Yanbo Chen, Miao Liu, Siyuan Wang · 发表于：Scientific Reports · 年份：2020 · DOI：10.1038/s41598-020-76439-x · 被引用次数：54 · 研究领域：Advanced biosensing and bioanalysis techniques、Advanced Fluorescence Microscopy Techniques、Advanced Biosensing Techniques and Applications

Fluorescence in situ hybridization (FISH) is a powerful method to visualize the spatial positions of specific genomic loci and RNA species. Recent technological advances have leveraged FISH to visualize these features in a highly multiplexed manner. Notable examples include chromatin tracing, RNA multiplexed error-robust FISH (MERFISH), multiplexed imaging of nucleome architectures (MINA), and sequential single-molecule RNA FISH. However, one obstacle to the broad adoption of these methods is the complexity of the multiplexed FISH probe design. In this paper, we introduce an easy-to-use, versatile, and all-in-one application called ProbeDealer to design probes for a variety of multiplexed FISH techniques and their combinations. ProbeDealer offers a one-stop shop for multiplexed FISH design needs of the research community.