Thiamine-Mediated Cooperation Between Auxotrophic Rhodococcus ruber ZM07 and Escherichia coli K12 Drives Efficient Tetrahydrofuran Degradation

作者：Hui Huang, Minbo Qi, Yiming Liam Liu, Haixia Wang, Xuejun Wang, Yiyang Qiu, Zhenmei Lü · 发表于：Frontiers in Microbiology · 年份：2020 · DOI：10.3389/fmicb.2020.594052 · 被引用次数：6 · 研究领域：Alcoholism and Thiamine Deficiency、Microbial Metabolic Engineering and Bioproduction、Amino Acid Enzymes and Metabolism

Tetrahydrofuran (THF) is a universal solvent widely used in the synthesis of chemicals and pharmaceuticals. As a refractory organic contaminant, it can only be degraded by a small group of microbes. In this study, a thiamine auxotrophic THF-degrading bacterium, Rhodococcus ruber ZM07, was isolated from an enrichment culture H-1. It was cocultured with Escherichia coli K12 (which cannot degrade THF but can produce thiamine) and/or Escherichia coli K12Δ thiE (which can neither degrade THF nor produce thiamine) with or without exogenous thiamine. This study aims to understand the interaction mechanisms between ZM07 and K12. We found that K12 accounted for 30% of the total when cocultured and transferred with ZM07 in thiamine-free systems; in addition, in the three-strain (ZM07, K12, and K12Δ thiE ) cocultured system without thiamine, K12Δ thiE disappeared in the 8th transfer, while K12 could still stably exist (the relative abundance remained at approximately 30%). The growth of K12 was significantly inhibited in the thiamine-rich system. Its proportion was almost below 4% after the fourth transfer in both the two-strain (ZM07 and K12) and three-strain (ZM07, K12, and K12Δ thiE ) systems; K12Δ thiE ’s percentage was higher than K12’s in the three-strain (ZM07, K12, and K12Δ thiE ) cocultured system with exogenous thiamine, and both represented only a small proportion (less than 1% by the fourth transfer). The results of the coculture of K12 and K12Δ thiE in thiamine-free medium ...