Hierarchical Self‐assembly of Discrete Metal–Organic Cages into Supramolecular Nanoparticles for Intracellular Protein Delivery

作者：Ji Liu, Tianli Luo, Yifei Xue, Lanqun Mao, Peter J. Stang, Ming Wang · 发表于：Angewandte Chemie International Edition · 年份：2020 · DOI：10.1002/anie.202013904 · 被引用次数：108 · 研究领域：Advanced biosensing and bioanalysis techniques、RNA Interference and Gene Delivery、Supramolecular Self-Assembly in Materials

Abstract Hierarchical self‐assembly (HAS) is a powerful approach to create supramolecular nanostructures for biomedical applications. This potency, however, is generally challenged by the difficulty of controlling the HAS of biomacromolecules and the functionality of resulted HAS nanostructures. Herein, we report a modular approach for controlling the HAS of discrete metal–organic cages (MOC) into supramolecular nanoparticles, and its potential for intracellular protein delivery and cell‐fate specification. The hierarchical coordination‐driven self‐assembly of adamantane‐functionalized M 12 L 24 MOC (Ada‐MOC) and the host–guest interaction of Ada‐MOC with β‐cyclodextrin‐conjugated polyethylenimine (PEI‐βCD) afford supramolecular nanoparticles in a controllable manner. HAS maintains high efficiency and orthogonality in the presence of protein, enabling the encapsulation of protein into the nanoparticles for intracellular protein delivery for therapeutic application and CRISPR/Cas9 genome editing.