Automating Cloning by Natural Transformation

作者：Xinglin Jiang, Emilia Palazzotto, Ewa Wybraniec, Lachlan Munro, Haibo Zhang, Douglas B. Kell, Tilmann Weber, Sang Yup Lee · 发表于：ACS Synthetic Biology · 年份：2020 · DOI：10.1021/acssynbio.0c00240 · 被引用次数：27 · 研究领域：CRISPR and Genetic Engineering、Bacterial Genetics and Biotechnology、Microbial Metabolic Engineering and Bioproduction

Affordable and automated cloning platforms are essential to many synthetic biology studies. However, the traditional E. coli -based cloning is a major bottleneck as it requires heat shock or electroporation implemented in the robotic workflows. To overcome this problem, we explored bacterial natural transformation for automatic DNA cloning and engineering. Recombinant plasmids are efficiently generated from Gibson or overlap extension PCR (OE-PCR) products by simply adding the DNA into Acinetobacter baylyi ADP1 cultures. No DNA purification, competence induction, or special equipment is required. Up to 10,000 colonies were obtained per microgram of DNA, while the number of false positive colonies was low. We cloned and engineered 21 biosynthetic gene clusters (BGCs) of various types, with length from 1.5 to 19 kb and GC content from 35% to 72%. One of them, a nucleoside BGC, showed antibacterial activity. Furthermore, the method was easily transferred to a low-cost benchtop robot with consistent cloning efficiency. Thus, this automatic natural transformation (ANT) cloning provides an easy, robust, and affordable platform for high throughput DNA engineering.