QTL analysis across multiple environments reveals promising chromosome regions associated with yield-related traits in maize under drought conditions

作者：Xinmin Hu, Guihua Wang, Guihua Wang, Xuemei Du, Hongwei Zhang, Zhenxiang Xu, Jie Wang, Jie Wang, Guo Chen, Bo Wang, Xuhui Li, Xunji Chen, Junjie Fu, Jun Zheng, Jianhua Wang, Jianhua Wang, Riliang Gu, Guoying Wang, Guoying Wang · 发表于：The Crop Journal · 年份：2020 · DOI：10.1016/j.cj.2020.10.004 · 被引用次数：35 · 研究领域：Genetic Mapping and Diversity in Plants and Animals、Genetics and Plant Breeding、Wheat and Barley Genetics and Pathology

Drought is one of the most critical abiotic stresses influencing maize yield. Improving maize cultivars with drought tolerance using marker-assisted selection requires a better understanding of its genetic basis. In this study, a doubled haploid (DH) population consisting of 217 lines was created by crossing the inbred lines Han 21 (drought-tolerant) and Ye 478 (drought-sensitive). The population was genotyped with a 6 K SNP assay and 756 SNP (single nucleotide polymorphism) markers were used to construct a linkage map with a length of 1344 cM. Grain yield (GY), ear setting percentage (ESP), and anthesis–silking interval (ASI) were recorded in seven environments under well-watered (WW) and water-stressed (WS) regimes. High phenotypic variation was observed for all traits under both water regimes. Using the LSMEAN (least-squares mean) values from all environments for each trait, 18 QTL were detected, with 9 associated with the WW and 9 with the WS regime. Four chromosome regions, Chr. 3: 219.8–223.7 Mb, Chr. 5: 191.5–194.7 Mb, Chr. 7: 132.2–135.6 Mb, and Chr. 10: 88.2–89.4 Mb, harbored at least 2 QTL in each region, and QTL co-located in a region inherited favorable alleles from the same parent. A set of 64 drought-tolerant BC3F6 lines showed preferential accumulation of the favorable alleles in these four regions, supporting an association between the four regions and maize drought tolerance. QTL-by-environment interaction analysis revealed 28 edQTL (environment-dependent QTL...