Assembly of the algal CO 2 -fixing organelle, the pyrenoid, is guided by a Rubisco-binding motif

作者：Moritz T. Meyer, Alan K. Itakura, Weronika Patena, Lianyong Wang, Shan He, Tom Z. Emrich‐Mills, Chun Sing Lau, Gary F. Yates, Luke C. M. Mackinder, Martin C. Jonikas · 发表于：Science Advances · 年份：2020 · DOI：10.1126/sciadv.abd2408 · 被引用次数：103 · 研究领域：Algal biology and biofuel production、Photosynthetic Processes and Mechanisms、Marine and coastal ecosystems

, we found that pyrenoid proteins share a sequence motif. We show that the motif is necessary and sufficient to target proteins to the pyrenoid and that the motif binds to Rubisco, suggesting a mechanism for targeting. The presence of the Rubisco-binding motif on proteins that localize to the tubules and on proteins that localize to the matrix-starch sheath interface suggests that the motif holds the pyrenoid's three subcompartments together. Our findings advance our understanding of pyrenoid biogenesis and illustrate how a single protein motif can underlie the architecture of a complex multilayered phase-separated organelle.