T-dependent B cell responses to Plasmodium induce antibodies that form a high-avidity multivalent complex with the circumsporozoite protein

作者：Camilla R. Fisher, Henry J. Sutton, Joe A. Kaczmarski, Hayley A. McNamara, Ben Clifton, Joshua A. Mitchell, Yeping Cai, Johanna Dups, Nicholas J. D'Arcy, Mandeep Singh, Aaron Chuah, Thomas S. Peat, Colin J. Jackson, Ian A. Cockburn · 发表于：bioRxiv (Cold Spring Harbor Laboratory) · 年份：2017 · DOI：10.1101/108746 · 被引用次数：3 · 研究领域：Malaria Research and Control、Complement system in diseases、vaccines and immunoinformatics approaches

Abstract The repeat region of the Plasmodium falciparum circumsporozoite protein (CSP) is a major vaccine antigen because it can be targeted by parasite neutralizing antibodies; however, little is known about this interaction. We used isothermal titration calorimetry, X-ray crystallography and mutagenesis-validated modeling to analyze the binding of a murine neutralizing antibody to Plasmodium falciparum CSP. Strikingly, we found that the repeat region of CSP is bound by multiple antibodies. This repeating pattern allows multiple weak interactions of single F AB domains to accumulate and yield a complex with a dissociation constant in the low nM range. Because the CSP protein can potentially cross-link multiple B cell receptors (BCRs) we hypothesized that the B cell response might be T cell independent. However, while there was a modest response in mice deficient in T cell help, the bulk of the response was T cell dependent. By sequencing the BCRs of CSP-repeat specific B cells in inbred mice we found that these cells underwent somatic hypermutation and affinity maturation indicative of a T-dependent response. Last, we found that the BCR repertoire of responding B cells was limited suggesting that the structural simplicity of the repeat may limit the breadth of the immune response. Author Summary Vaccines aim to protect by inducing the immune system to make molecules called antibodies that can recognize molecules on the surface of invading pathogens. In the case of malaria, o...