Systemic elucidation on the potential bioactive compounds and hypoglycemic mechanism of Polygonum multiflorum based on network pharmacology

作者：Yunfei Song, Jianbo Yang, Wenguang Jing, Qi Wang, Yue Liu, Xian‐Long Cheng, Fei Ye, Jin‐Ying Tian, Feng Wei, Shuang‐Cheng Ma · 发表于：Chinese Medicine · 年份：2020 · DOI：10.1186/s13020-020-00401-2 · 被引用次数：31 · 研究领域：Phytochemistry and biological activity of medicinal plants、Natural Antidiabetic Agents Studies、Mangiferin and Mango Extracts

BACKGROUND: Diabetes is a complex metabolic disease characterized by hyperglycemia, plaguing the whole world. However, the action mode of multi-component and multi-target for traditional Chinese medicine (TCM) could be a promising treatment of diabetes mellitus. According to the previous research, the TCM of Polygonum multiflorum (PM) showed noteworthy hypoglycemic effect. Up to now, its hypoglycemic active ingredients and mechanism of action are not yet clear. In this study, network pharmacology was employed to elucidate the potential bioactive compounds and hypoglycemic mechanism of PM. METHODS: First, the compounds with good pharmacokinetic properties were screened from the self-established library of PM, and the targets of these compounds were predicted and collected through database. Relevant targets of diabetes were summarized by searching database. The intersection targets of compound-targets and disease-targets were obtained soon. Secondly, the interaction net between the compounds and the filtered targets was established. These key targets were enriched and analyzed by protein-protein interactions (PPI) analysis, molecular docking verification. Thirdly, the key genes were used to find the biologic pathway and explain the therapeutic mechanism by genome ontology (GO) and kyoto encyclopedia of genes and genomes (KEGG) analysis. Lastly, the part of potential bioactive compounds were under enzyme activity inhibition tests. RESULTS: In this study, 29 hypoglycemic componen...