[Evodiamine Promotes Apoptosis of Glioma SHG-44 Cells and Its Mechanism].

作者：Lu Liu, Xuemei Wang, Yan Wang, Hongmei Xi, Jianduo Guo, Qiujin Huang · 发表于：PubMed · 年份：2020 · DOI：10.3881/j.issn.1000-503x.11853 · 被引用次数：3 · 研究领域：Quinazolinone synthesis and applications、Synthesis and Biological Evaluation、Synthesis and biological activity

cultured glioma SHG-44 cells were divided into control group and evodiamine group(which was further divided into three subgroups according to the glycoside concentrations).Cell viability was determined by CCK-8 method,cells apoptosis rate by flow cytometry,and nucleus apoptosis by Hoechst 33258 nuclear staining.Cell morphological changes were observed by transmission electron microscope.Protein expressions of Cleaved Caspase-3 and Cleaved Caspase-9 were detected by Western blot analysis.Results Evodiamine significantly inhibited the proliferation of glioma SHG-44 cells.The apoptosis rate of Glioma cells increased in a dose-dependent manner as the evodiamine concentration increased.Evodiamine promoted the expressions of cleaved Caspase-3 and cleaved Caspase-9.Conclusion Evodiamine inhibits glioma cell proliferation by changing the expressions of cleaved Caspase-3 and cleaved Caspase-9.