Staphyloccocus aureus and Staphyloccocus epidermidis: Biofilm development on different cobalt-chrome alloys for implant materials in orthopedy

作者：Birgit Lohberger, Benjamin Bödendorfer, Lukas Wolrab, Astrid H. Paulitsch‐Fuchs · 发表于：Zeitschrift für Orthopädie und Unfallchirurgie · 年份：2020 · DOI：10.1055/s-0040-1717266 · 研究领域：Bone Tissue Engineering Materials、Orthopedic Infections and Treatments、Orthopaedic implants and arthroplasty

Objectives Peri-prosthetic infections (PIs), caused by the adhesion of bacteria to medical implant materials, are among the most serious complications which can occur following joint replacement surgeries. The bacteria form biofilms on the surfaces, triggering inflammatory reactions in the tissue surrounding the periprosthetic area. Those infectious reactions occur mostly within the first two prosthetic years and can results in the rejection of the implant or, in the worst case, in amputations. The most common pathogens in this formation are coagulase-negative Staphylococcus spp. (up to 43%), Staphylococcus aureus (up to 23%), Streptococcus spp. (9-10%), as well as gram-negative rods or Enterococci. The basic aim of the here presented work was to understand which casting alloys have the worst properties for the adhesion of infectious microorganisms - specifically S. aureus and S. epidermidis. For this purpose, six different cobalt-chromium-molybdenum (CoCrMo) alloys were tested: CoCrMo, “CoCrMo TiN (titanium nitride)”, “CoCrMo porous coated”, “CoCrMo cpTi (commercially pure titanium)”, “CoCrMo cpTi + TCP (tricalcium phosphate) and “CoCrMo highly-polished.” Methods For biofilm preparation, 24 well plates were prepared by filling them with four platelets per alloy type. After adding 1.5 mL of the respective cell suspension (S. aureus or S. epidermidis in a cell density of McFarland 0.5) into each well, the well plates were sealed with a breathable membrane and incubated on the ...