Identification of fruit size associated quantitative trait loci featuring SLAF based high-density linkage map of goji berry (Lycium spp.)

作者：Fazal Rehman, Haiguang Gong, Zhong Li, Shaohua Zeng, Tianshun Yang, Peiyan Ai, Lizhu Pan, Hongwen Huang, Ying Wang · 发表于：BMC Plant Biology · 年份：2020 · DOI：10.1186/s12870-020-02567-1 · 被引用次数：45 · 研究领域：Polysaccharides and Plant Cell Walls、Plant Gene Expression Analysis、Genetic Mapping and Diversity in Plants and Animals

Abstract Background Goji ( Lycium spp., 2n = 24) is a fruit bearing woody plant popular as a superfood for extensive medicinal and nutritional advantages. Fruit size associated attributes are important for evaluating small-fruited goji berry and plant architecture. The domestication traits are regulated quantitatively in crop plants but few studies have attempted on genomic regions corresponding to fruit traits. Results In this study, we established high-resolution map using specific locus amplified fragment (SLAF) sequencing for de novo SNPs detection based on 305 F 1 individuals derived from L. chinense and L. barbarum and performed quantitative trait loci (QTL) analysis of fruit size related traits in goji berry. The genetic map contained 3495 SLAF markers on 12 LGs, spanning 1649.03 cM with 0.47 cM average interval. Female and male parents and F 1 individuals` sequencing depth was 111.85-fold and 168.72-fold and 35.80-fold, respectively. The phenotype data were collected for 2 successive years (2018–2019); however, two-year mean data were combined in an extra year (1819). Total 117 QTLs were detected corresponding to multiple traits, of which 78 QTLs in 2 individual years and 36 QTLs in extra year. Six Promising QTLs ( qFW10–6.1 , qFL10–2.1 , qLL10–2.1 , qLD10–2.1 , qLD12–4.1 , qLA10–2.1 ) were discovered influencing fruit weight, fruit length and leaf related attributes covering an interval ranged from 27.32–71.59 cM on LG10 with peak LOD of 10.48 and 14.6% PVE. Three QT...