Multiple omics analysis reveals that high fiber diets promote gluconeogenesis and inhibit glycolysis in muscle

作者：Jianghong Wu, Yang Ding, Husile Gong, Yunxia Qi, Hailian Sun, Yongbin Liu, Yahong Liu, Qiu Xiao · 发表于：BMC Genomics · 年份：2020 · DOI：10.1186/s12864-020-07048-1 · 被引用次数：19 · 研究领域：Ruminant Nutrition and Digestive Physiology、Meat and Animal Product Quality、Genetic and phenotypic traits in livestock

BACKGROUND: Meat quality is a complex trait affected by genotypic and environmental factors. In a previous study, it was found that feedstuffs have various effects on the growth rate and meat quality of lambs. However, the underlying mechanisms are still not entirely clear. RESULTS: In this study, to investigate the mechanisms that impact meat quality in twin sheep fed either with high fiber low protein (HFLP) forage (Ceratoides) or low fiber high protein (LFHP) forage (alfalfa) diets, multi omics techniques were utilized for integration analysis based on the feed nutritional value and the sheep microbiome, transcriptome, metabolome, and fatty acid profile. Results showed that the production performance and the muscle components of lambs were significantly affected by feeds. The essential fatty acid (linoleic acid and arachidonic acid) content of the muscle, based on gas chromatography-mass spectrometry analysis, was increased when lambs were fed with HFLP. The microbes in the lambs' rumen fed a HFLP diet were more diverse than those of the LFHP fed group. Besides, the ratio of Bacteroidetes and Firmicutes in the rumen of the sheep fed a LFHP diet was 2.6 times higher than that of the HFLP fed group. Transcriptome analysis of the muscle revealed that the genes related to glucose metabolic processes and fatty acid biosynthesis were significantly differentially expressed between the two groups. Potential cross talk was found between the sfour omics data layers, which helps to u...