Melting dsDNA Donor Molecules Greatly Improves Precision Genome Editing in Caenorhabditis elegans

作者：Krishna Sumanth Ghanta, Craig C. Mello · 发表于：Genetics · 年份：2020 · DOI：10.1534/genetics.120.303564 · 被引用次数：274 · 研究领域：CRISPR and Genetic Engineering、Genetics, Aging, and Longevity in Model Organisms、Evolution and Genetic Dynamics

Abstract Melting and fast cooling double stranded DNA donor molecules prior to injection dramatically increases the frequency of homology-directed repair for edits such as insertions of fluorescent protein markers in Caenorhabditis elegans. Strategies described here enable consistently .....