Meiotic crossover reduction by virus‐induced gene silencing enables the efficient generation of chromosome substitution lines and reverse breeding in Arabidopsis thaliana

作者：Vanesa Calvo‐Baltanás, Cris L. Wijnen, Chao Yang, Nina I. Lukhovitskaya, C. Bastiaan de Snoo, Linus Hohenwarter, Joost J. B. Keurentjes, Hans de Jong, Arp Schnittger, T.G. Wijnker · 发表于：The Plant Journal · 年份：2020 · DOI：10.1111/tpj.14990 · 被引用次数：11 · 研究领域：Plant Virus Research Studies、Chromosomal and Genetic Variations、CRISPR and Genetic Engineering

Summary Plant breeding applications exploiting meiotic mutant phenotypes (like the increase or decrease of crossover (CO) recombination) have been proposed over the last years. As recessive meiotic mutations in breeding lines may affect fertility or have other pleiotropic effects, transient silencing techniques may be preferred. Reverse breeding is a breeding technique that would benefit from the transient downregulation of CO formation. The technique is essentially the opposite of plant hybridization: a method to extract parental lines from a hybrid. The method can also be used to efficiently generate chromosome substitution lines (CSLs). For successful reverse breeding, the two homologous chromosome sets of a heterozygous plant must be divided over two haploid complements, which can be achieved by the suppression of meiotic CO recombination and the subsequent production of doubled haploid plants. Here we show the feasibility of transiently reducing CO formation using virus‐induced gene silencing (VIGS) by targeting the meiotic gene MSH5 in a wild‐type heterozygote of Arabidopsis thaliana . The application of VIGS (rather than using lengthy stable transformation) generates transgene‐free offspring with the desired genetic composition: we obtained parental lines from a wild‐type heterozygous F 1 in two generations. In addition, we obtained 20 (of the 32 possible) CSLs in one experiment. Our results demonstrate that meiosis can be modulated at will in A. thaliana to generate C...