A V H 1-69 antibody lineage from an infected Chinese donor potently neutralizes HIV-1 by targeting the V3 glycan supersite

作者：Sonu Kumar, Bin Ju, Benjamin G. Shapero, Xiaohe Lin, Li Ren, Lei Zhang, Dan Li, Zehua Zhou, Yi Feng, Cindy Sou, Colin Mann, Yanling Hao, Anita Sarkar, Jiali Hou, Christian Nunnally, Kunxue Hong, Shuo Wang, Xiangyang Ge, Bin Su, Elise Landais, Devin Sok, Michael B. Zwick, Linling He, Jiang Zhu, Ian A. Wilson, Yiming Shao · 发表于：Science Advances · 年份：2020 · DOI：10.1126/sciadv.abb1328 · 被引用次数：31 · 研究领域：HIV Research and Treatment、HIV/AIDS drug development and treatment、Cytomegalovirus and herpesvirus research

1-69 germline gene, and have a disulfide-linked long HCDR3 loop. Crystal structures of Env-bound and unbound antibodies revealed heavy chain-mediated recognition of the glycan supersite with a unique angle of approach and a critical role of the intra-HCDR3 disulfide. The mechanism of viral escape was examined via single-genome amplification/sequencing and glycan mutations around the N332 supersite. Our findings further emphasize the V3 glycan supersite as a prominent target for Env-based vaccine design.