Resveratrol alleviates the interleukin-1β-induced chondrocytes injury through the NF-κB signaling pathway

作者：Hong Yi, Wei Zhang, Zhiming Cui, Shengyu Cui, Jianbo Fan, Xinhui Zhu, Wei Liu · 发表于：Journal of Orthopaedic Surgery and Research · 年份：2020 · DOI：10.1186/s13018-020-01944-8 · 被引用次数：45 · 研究领域：Osteoarthritis Treatment and Mechanisms、Sirtuins and Resveratrol in Medicine、Rheumatoid Arthritis Research and Therapies

BACKGROUND: Osteoarthritis (OA) is a regular age-related disease that affects millions of people. Resveratrol (RSV) is a flavonoid with a stilbene structure with different pharmacological effects. The purpose of the experiment was to evaluate the protective role of RSV against the human OA chondrocyte injury induced by interleukin-1β (IL-1β). METHODS: Chondrocytes were isolated from OA patients and identified by type II collagen, safranin O staining, and toluidine blue staining. Differentially expressed genes in chondrocytes treated RSV were identified by RNA sequencing. Kyoto encyclopedia of genes and genomes (KEGG) pathway as well as gene ontology (GO) were further conducted through Metascape online tool. A cell counting kit-8 (CCK-8) assay was applied to discover the viability of chondrocytes (6, 12, 24, and 48 μM). Many genes associated with inflammation and matrix degradation are evaluated by real-time PCR (RT-PCR) as well as western blot (WB). The mechanism of RSV for protecting IL-1β induced chondrocytes injury was further measured through immunofluorescence and WB assays. RESULTS: A total of 845 differentially expressed genes (upregulated = 499, downregulated = 346) were found. These differentially expressed genes mainly enriched into negative regulation of catabolic process, autophagy, and cellular catabolic process, intrinsic apoptotic, apoptotic, and regulation of apoptotic signaling pathway, cellular response to abiotic stimulus, external stimuli, stress, and radi...