Grain Size Selection Using Novel Functional Markers Targeting 14 Genes in Rice

作者：Lin Zhang, Bin Ma, Zhong Bian, Xiaoyuan Li, Changquan Zhang, Jiyun Liu, Qun Li, Qiaoquan Liu, Zuhua He · 发表于：Rice · 年份：2020 · DOI：10.1186/s12284-020-00427-y · 被引用次数：42 · 研究领域：Genetic Mapping and Diversity in Plants and Animals、GABA and Rice Research、Rice Cultivation and Yield Improvement

Abstract Background Grain size is an extremely important aspect of rice breeding, affecting both grain yield and quality traits. It is controlled by multiple genes and tracking these genes in breeding schemes should expedite selection of lines with superior grain yield and quality, thus it is essential to develop robust, efficient markers. Result In this study, 14 genes related to grain size ( GW2 , GS2 , qLGY3 , GS3 , GL3.1 , TGW3 , GS5 , GW5 , GS6 , TGW6 , GW6a , GLW7 , GL7 and GW8 ) were selected for functional marker development. Twenty-one PCR-gel-based markers were developed to genotype the candidate functional nucleotide polymorphisms (FNPs) of these genes, and all markers can effectively recognize the corresponding allele types. To test the allele effects of different FNPs, a global collection of rice cultivars including 257 accessions from the Rice Diversity Panel 1 was used for allele mining, and four grain-size-related traits were investigated at two planting locations. Three FNPs for GW2 , GS2 and GL3.1 were genotyped as rare alleles only found in cultivars with notably large grains, and the allele contributions of the remaining FNPs were clarified in both the indica and japonica subspecies. Significant trait contributions were found for most of the FNPs, especially GS3 , GW5 and GL7 . Of note, GW5 could function as a key regulator to coordinate the performance of other grain size genes. The allele effects of several FNPs were also tested by QTL analysis using an ...