The Peak Interval Procedure in Rodents: A Tool for Studying the Neurobiological Basis of Interval Timing and Its Alterations in Models of Human Disease

作者：Fuat Balcı, David Freestone · 发表于：BIO-PROTOCOL · 年份：2020 · DOI：10.21769/bioprotoc.3735 · 被引用次数：14 · 研究领域：Neuroscience and Music Perception、Neural dynamics and brain function、Diverse Music Education Insights

Animals keep track of time intervals in the seconds to minutes range with, on average, high accuracy but substantial trial-to-trial variability. The ability to detect the statistical signatures of such timing behavior is an indispensable feature of a good and theoretically-tractable testing procedure. A widely used interval timing procedure is the peak interval (PI) procedure, where animals learn to anticipate rewards that become available after a fixed delay. After learning, they cluster their responses around that reward-availability time. The in-depth analysis of such timed anticipatory responses leads to the understanding of an internal timing mechanism, that is, the processing dynamics and systematic biases of the brain’s clock. This protocol explains in detail how the PI procedure can be implemented in rodents, from training through testing to analysis. We showcase both trial-by-trial and trial-averaged analytical methods as a window into these internal processes. This protocol has the advantages of capturing timing behavior in its full-complexity in a fashion that allows for a theoretical treatment of the data., [摘要] 动物跟踪秒至分钟范围内的时间间隔，平均而言具有很高的准确性，但审判与审判之间存在很大差异。检测此类计时行为的统计特征的能力是良好且在理论上易于处理的测试过程的必不可少的特征。广泛使用的间隔计时程序是峰值间隔（PI）程序，在此程序中，动物学会预测固定的延迟后可获得的奖励。学习后，他们会在奖励可用时间将他们的响应集中在周围。对此类定时预期响应的深入分析导致人们了解了内部定时机制，即大脑时钟的处理动力学和系统性偏差。Ť 他的协议详细解释了从培训到测试再到分析，如何在啮齿动物中实施PI程序。我们展示了逐次试验和平均试验分析方法，作为这些内部过程的窗口。该协议的优势在于，它可以以一种完全复杂的方式捕获时序行为，从而可以对数据进行理论上的处理。[背景] Ø rganisms 感知时间，就好像它是一个感觉。它遵...