Simultaneous Detection of Three Foodborne Pathogens Based on Immunomagnetic Nanoparticles and Fluorescent Quantum Dots

作者：Dan Wang, Fengnan Lian, Shuo Yao, Yi Liu, Jinpeng Wang, Xiuling Song, Lirui Ge, Yue Wang, Yuyi Zhao, Jiamei Zhang, Chao Zhao, Kun Xu · 发表于：ACS Omega · 年份：2020 · DOI：10.1021/acsomega.0c02833 · 被引用次数：45 · 研究领域：Biosensors and Analytical Detection、Enterobacteriaceae and Cronobacter Research、Vibrio bacteria research studies

High Resolution Image Download MS PowerPoint Slide This paper presents a peptide-mediated immunomagnetic separation technique and an immunofluorescence quantum dot technique for simultaneous and rapid detection of Escherichia coli O157:H7, Staphylococcus aureus, and Vibrio parahaemolyticus . First, three peptides that can specifically recognize the three foodborne pathogens were combined with magnetic nanoparticles to form an immunomagnetic nanoparticle probe for capturing three kinds of target bacteria and then added three quantum dot probes (quantum dots-aptamer), which formed a sandwich composite structure. When the three quantum dot probes specifically combined with the three pathogenic bacteria, the remaining fluorescent signal in the supernatant will be reduced by magnetic separation. Therefore, the remaining fluorescent signal in the supernatant can be measured with a fluorescence spectrophotometer to indirectly determine the three pathogens in the sample. The linear range of the method was 10–10 7 cfu/mL, and in the buffer, the detection limits of E. coli O157:H7, S. aureus, and V. parahaemolyticus were 2.460, 5.407, and 3.770 cfu/mL, respectively. In the tap water simulation, the detection limits of E. coli O157:H7, S. aureus, and V. parahaemolyticus were 2.730, 1.990 × 10 1, and 4.480 cfu/mL, respectively. In the milk simulation sample, the detection limits of E. coli O157:H7, S. aureus, and V. parahaemolyticus were 6.660, 1.070 × 10 1, and 2.236 × 10 1 cfu/mL, resp...