Flippases play specific but distinct roles in the development, pathogenicity, and secondary metabolism of Fusarium graminearum

作者：Yingzi Yun, Pusheng Guo, Jing Zhang, Haixia You, Pingting Guo, Huobin Deng, Yixin Hao, Limei Zhang, Xueyu Wang, Yakubu Saddeeq Abubakar, Jie Zhou, Guodong Lu, Zonghua Wang, Wenhui Zheng · 发表于：Molecular Plant Pathology · 年份：2020 · DOI：10.1111/mpp.12985 · 被引用次数：23 · 研究领域：Mycotoxins in Agriculture and Food、Plant-Microbe Interactions and Immunity、Plant Pathogens and Fungal Diseases

The membrane trafficking system is important for compartmentalization of the biosynthesis pathway and secretion of deoxynivalenol (DON) mycotoxin (a virulence factor) in Fusarium graminearum. Flippases are transmembrane lipid transporters and mediate a number of essential physiological steps of membrane trafficking, including vesicle budding, charging, and protein diffusion within the membrane. However, the roles of flippases in secondary metabolism remain unknown in filamentous fungi. Herein, we identified five flippases (FgDnfA, FgDnfB, FgDnfC1, FgDnfC2, and FgDnfD) in F. graminearum and established their specific and redundant functions in the development and pathogenicity of this phytopathogenic fungus. Our results demonstrate that FgDnfA is critical for normal vegetative growth while the other flippases are dispensable. FgDnfA and FgDnfD were found crucial for the fungal pathogenesis, and a remarkable reduction in DON production was observed in ΔFgDNFA and ΔFgDNFD. Deletion of the FgDNFB gene increased DON production to about 30 times that produced by the wild type. Further analysis showed that FgDnfA and FgDnfD have positive roles in the regulation of trichothecene (TRI) genes (TRI1, TRI4, TRI5, TRI6, TRI12, and TRI101) expression and toxisome reorganization, while FgDnfB acts as a negative regulator of DON synthesis. In addition, FgDnfB and FgDnfD have redundant functions in the regulation of phosphatidylcholine transport, and double deletion of FgDNFB and FgDNFD showe...