Cigarillos Compromise the Mucosal Barrier and Protein Expression in Airway Epithelia

作者：Sabri H. Abdelwahab, Boris Reidel, Jessica R. Martin, Arunava Ghosh, James E. Keating, Prashamsha Haridass, Jerome Carpenter, Gary L. Glish, Robert Tarran, Claire M. Doerschuk, Mehmet Kesımer · 发表于：American Journal of Respiratory Cell and Molecular Biology · 年份：2020 · DOI：10.1165/rcmb.2019-0085oc · 被引用次数：5 · 研究领域：Invertebrate Immune Response Mechanisms、Antimicrobial Peptides and Activities、Asthma and respiratory diseases

Abstract Smoking remains a leading cause of preventable morbidity and mortality worldwide. Despite a downward trend in cigarette use, less-regulated tobacco products, such as cigarillos, which are often flavored to appeal to specific demographics, such as younger people, are becoming increasingly popular. Cigar/cigarillo smoking has been considered a safer alternative to cigarettes; however, the health risks associated with cigar in comparison with cigarette smoking are not well understood. To address this knowledge gap, we characterized the effects of multiple brands of cigarillos on the airway epithelium using ex vivo and in vivo models. To analyze these effects, we assessed the cellular viability and integrity of smoke-exposed primary airway cell cultures. We also investigated the protein compositions of apical secretions from cigarillo-exposed airway epithelial cultures and BAL fluid of cigarillo-exposed mice through label-free quantitative proteomics and determined the chemical composition of smoke collected from the investigated cigarillo products. We found that cigarillo smoke exerts similar or greater effects than cigarette smoke in terms of reduced cell viability; altered protein levels, including those of innate immune proteins; induced oxidative-stress markers; and greater nicotine delivery to cells. The analysis of the chemical composition of the investigated cigarillo products revealed differences that might be linked to the differential effects of these products...