Monoamine Oxidase A is a Major Mediator of Mitochondrial Homeostasis and Glycolysis in Gastric Cancer Progression

作者：Ling Chen, Li Guo, Ziwen Sun, Guochun Yang, Jing Guo, Kai Chen, Ruixue Xiao, Yang Xigui, Lijun Sheng · 发表于：Cancer Management and Research · 年份：2020 · DOI：10.2147/cmar.s257848 · 被引用次数：47 · 研究领域：Microbial metabolism and enzyme function、Amino Acid Enzymes and Metabolism、Tryptophan and brain disorders

OBJECTIVE: Monoamine oxidase A (MAO-A) is a mitochondrial protein involved in tumourigenesis in different types of cancer. However, the biological function of MAO-A in gastric cancer development remains unknown. METHODS: We examined MAO-A expression in gastric cancer tissues and in gastric cancer cell lines by immunohistochemistry and Western blot analyses. CCK8, FACS and bromodeoxyuridine incorporation assays were performed to assess the effects of MAO-A on gastric cancer cell proliferation. The role of MAO-A in mitochondrial function was determined through MitoSOX Red staining, ATP generation and glycolysis assays. RESULTS: In the present study, we observed that MAO-A was significantly upregulated in gastric cancer tissues and in AGS and MGC803 cells. The observed MAO-A inhibition indicated decreased cell cycle progression and proliferation. Silencing MAO-A expression was associated with suppressed migration and invasion of gastric cancer cells in vitro. Moreover, alleviated mitochondrial damage in these cells was demonstrated by decreased levels of mitochondrial reactive oxygen species and increased ATP generation. MAO-A knockdown also regulated the expression of the glycolysis rate-limiting enzymes hexokinase 2 and pyruvate dehydrogenase. Finally, we observed that the glycolysis-mediated effect was weakened in AGS and MGC803 cells when MAO-A was blocked. CONCLUSION: The findings of the present study indicate that MAO-A is responsible for mitochondrial dysfunction and aero...