PCR Past, Present and Future

作者：Hanliang Zhu, Haoqing Zhang, Ying Xu, Soňa Laššáková, Marie Korabečná, Pavel Neužil · 发表于：BioTechniques · 年份：2020 · DOI：10.2144/btn-2020-0057 · 被引用次数：379 · 研究领域：Molecular Biology Techniques and Applications、Biosensors and Analytical Detection、Innovative Microfluidic and Catalytic Techniques Innovation

PCR has become one of the most valuable techniques currently used in bioscience, diagnostics and forensic science. Here we review the history of PCR development and the technologies that have evolved from the original PCR method. Currently, there are two main areas of PCR utilization in bioscience: high-throughput PCR systems and microfluidics-based PCR devices for point-of-care (POC) applications. We also discuss the commercialization of these techniques and conclude with a look into their modifications and use in innovative areas of biomedicine. For example, real-time reverse transcription PCR is the gold standard for SARS-CoV-2 diagnoses. It could also be used for POC applications, being a key component of the sample-to-answer system.