miRNA-425-5p enhances lung cancer growth via the PTEN/PI3K/AKT signaling axis

作者：Jin-shan Zhou, Ze-shan Yang, Siyang Cheng, Jianghao Yu, Chaojun Huang, Qiang Feng · 发表于：BMC Pulmonary Medicine · 年份：2020 · DOI：10.1186/s12890-020-01261-0 · 被引用次数：52 · 研究领域：MicroRNA in disease regulation、PI3K/AKT/mTOR signaling in cancer、Circular RNAs in diseases

BACKGROUND: miRNAs regulate a multitude of cellular processes and their aberrant regulation is linked to human cancer. However, the role of miR-425-5p in lung cancer (LCa) is still largely unclear. Here, we explored the role of miR-425-5p during LCa tumorigenesis. METHODS: Cell proliferation was evaluated by cell counting Kit-8 and colony formation assay. Western blot and real-time PCR were accordingly used to detect the relevant proteins, miRNA and gene expression. Luciferase reporter assays were used to illustrate the interaction between miR-425-5p and PTEN. RESULTS: We demonstrate that miR-425-5p is overexpressed in LCa tissue and enhances the proliferative and colony formation capacity of the LCa cell lines A549 and NCI-H1299. Through predictive binding assays, PTEN was identified as a direct gene target and its exogenous expression inhibited the pro-cancer effects of miR-425-5p. Through its ability to down-regulate PTEN, miR-425-5p activated the PI3K/AKT axis. CONCLUSION: We conclude that miR-425-5p promotes LCa tumorigenesis through PTEN/PI3K/AKT signaling.