A non-human primate model of familial Alzheimer’s disease

作者：K. Sato, Hiroki Sasaguri, Wakako Kumita, Takashi Inoue, Yoko Kurotaki, Kenichi Nagata, Naomi Mihira, Kaori Sato, Tetsushi Sakuma, Takashi Yamamoto, Michihira Tagami, Ri‐Ichiroh Manabe, Kokoro Ozaki, Yasushi Okazaki, Takaomi C. Saido, Erika Sasaki · 发表于：bioRxiv (Cold Spring Harbor Laboratory) · 年份：2020 · DOI：10.1101/2020.08.24.264259 · 被引用次数：34 · 研究领域：Alzheimer's disease research and treatments、Cholinesterase and Neurodegenerative Diseases、Neuroscience and Neuropharmacology Research

Summary Alzheimer’s disease (AD) is a major cause of dementia, with the number of patients with this condition anticipated to exceed 50 million worldwide in the near future. Despite extensive research efforts, no effective measures are available to facilitate the prevention or treatment of AD, which is due in part to a lack of animal models able to closely replicate a human-like disease state. Here, we describe the generation of three mutant marmoset individuals in which exon 9 of PSEN1 gene product has been deleted ( PSEN1 -ΔE9). Such ΔE9 mutations have been reported to cause early on-set familial AD (references 1–5 ). We used Transcription Activator-Like Effector Nuclease (TALEN) to destroy the 3’ splice site of exon 9 in the marmoset PSEN1 gene. To this end, TALEN exhibits high genome-editing efficacy, generates few off-target effects, and produces minimal mosaicism. Indeed, whole genome sequencing and other analyses illustrated an absence of off-target effects and an apparent absence of mosaicism. Fibroblasts obtained from newborn marmosets exhibited uncleaved full-length presenilin 1 protein (PS1) caused by the perturbation of PS1 endoproteolysis as well as an increased ratio of Aβ 42 /Aβ 40 production, a signature of familial AD pathogenesis. To our knowledge, this is the first non-human primate model of familial AD. We intend to make our marmoset model available to the research community to facilitate the global fight against AD.