Simultaneous radial and wavelength analysis with the Optima XL-A analytical ultracentrifuge

作者：Peter Schuck · 发表于：Steinkopff eBooks · 年份：2007 · DOI：10.1007/bfb0115597 · 被引用次数：54 · 研究领域：Spectroscopy and Quantum Chemical Studies、Protein Interaction Studies and Fluorescence Analysis、Analytical Chemistry and Chromatography

We exploit the possibility offered by the new analytical ultracentrifuge Beckman Optima XL-A to record wavelength profiles at different radial positions and radial profiles at different wavelengths in sedimentation equilibrium experiments to unravel complexes formed by entities with different absorption spectra. The mathematical analysis is described here in detail. With a little a priori knowledge about the absorption spectra it is possible to resolve a greater number of components than with conventional methods. This method also enables one to achieve a simultaneous detection of the components’ extinction profiles. In addition, an analysis of each component’s statistical accuracy is presented.