Nanoscaled Bionic Periosteum Orchestrating the Osteogenic Microenvironment for Sequential Bone Regeneration

作者：Hanwen Li, Huan Wang, Jun Pan, Jiaying Li, Kai Zhang, Weifeng Duan, Huan Liang, Kangwu Chen, Dechun Geng, Qin Shi, Huilin Yang, Bin Li, Hao Chen · 发表于：ACS Applied Materials & Interfaces · 年份：2020 · DOI：10.1021/acsami.0c06906 · 被引用次数：68 · 研究领域：Bone Tissue Engineering Materials、Graphene and Nanomaterials Applications、Nanoplatforms for cancer theranostics

Periosteum orchestrates bone repair. Previously developed artificial periosteum was mainly focusing on materials modification to simply enhance bone formation, but few were attempting to make the artificial periosteum fit different bone repair stages. Here, we constructed a functionalized periosteum, which was composed of an electrospun scaffold grafted with leptin receptor antibody (LepR-a) and BMP2-loaded hollow MnO 2 (h-MnO 2 ) nanoparticles through a polydopamine (PDA)-assisted technique. The bionic periosteum showed suitable mechanical properties and favorable biocompatibility. It effectively recruited skeletal stem cells (SSCs) through antigen–antibody interactions, as in in vitro cell adhesion tests, we observed that more SSCs attached to the LepR-a-grafted periosteum compared to the control group. In vivo, the LepR-a-grafted periosteum covered on the cranial defect in Prx1-Cre/ERT2, -EGFP mice recruited more Prx1-EGFP cells to the fracture site compared to control groups at post-surgery day 3, 7, and 14. Co-staining with Sp7 indicated that most of the recruited Prx1-EGFP cells underwent osteogenic lineage commitment. Sustained BMP2 release from h-MnO 2 promoted osteogenesis by accelerating the osteogenic differentiation of recruited SSCs, as demonstrated by alkaline phosphatase (ALP) and alizarin red staining (ARS) in vitro and microcomputed tomography (micro-CT) in vivo . Interestingly, we also observed the growth of osteogenic coupled capillaries (CD31 hi Emcn hi ) ...