Circulating miRNA‐3552 as a Potential Biomarker for Ischemic Stroke in Rats

作者：Litao Li, Lipeng Dong, Jingru Zhao, Weiliang He, Bao Chu, Jijie Zhang, Zongkai Wu, Congying Zhao, Jinming Cheng, Wentao Yao, Hebo Wang · 发表于：BioMed Research International · 年份：2020 · DOI：10.1155/2020/4501393 · 被引用次数：13 · 研究领域：MicroRNA in disease regulation、Neuroinflammation and Neurodegeneration Mechanisms、Cancer-related molecular mechanisms research

Objective . With the growing incidence of ischemic stroke worldwide, there is an urgent need to identify blood biomarkers for ischemic stroke patients. Thus, our aim was to identify potential circulating microRNA (miRNA) as a potential biomarker and to explore its potential mechanism for ischemic stroke in rats. Methods. The mRNA dataset GSE97537 and miRNA dataset GSE97532 were downloaded from the Gene Expression Omnibus (GEO) GSE97537 including 7 middle cerebral artery occlusion (MCAO) rat brain tissues and 5 sham‐operated rat brain tissues GSE97532 including 6 MCAO rat blood samples and 3 sham‐operated rat blood samples. Differentially expressed mRNAs and miRNAs with corrected p value ≤ 0.01 and fold change ≥2 or ≤0.05 were identified. To explore potential biological processes and pathways of differentially expressed mRNAs, functional enrichment analysis was performed. The target mRNAs of differentially expressed miRNAs were predicted using DNA Intelligent Analysis (DIANA)‐microT tools. The target mRNAs and differentially expressed mRNAs were intersected. Results . 1228 differentially expressed mRNAs in MCAO rat brain tissues were identified. Highly expressed mRNAs were mainly enriched in the inflammatory responses. Nine differentially expressed miRNAs were identified in MCAO rat blood samples. A total of 673 target mRNAs were predicted to significantly bind these differentially expressed miRNAs. Among them, 54 target mRNAs were differentially expressed in MCAO rat blood sa...