Cell-free biology using remote-controlled digital microfluidics for individual droplet control

作者：Dong Liu, Zhenghuan Yang, Luyang Zhang, Minglun Wei, Yuan Lu · 发表于：RSC Advances · 年份：2020 · DOI：10.1039/d0ra04588h · 被引用次数：22 · 研究领域：Electrowetting and Microfluidic Technologies、Innovative Microfluidic and Catalytic Techniques Innovation、Microfluidic and Bio-sensing Technologies

remote real-time control inspired by the Internet of Things (IoT) was developed for detecting glucose enzyme catalytic cell-free reactions and verifying the feasibility of programmed cell-free protein expression. A cell-free biological reaction process which can be remote-controlled visually with excellent interactivity, controllability and flexibility was achieved. As proof-of-concept research, this work proposed a new control interface for single-drop cell-free biological reactions. It is much like the "droplet operation desktop" concept, used for remote-controllable operations and distributions of cell-free biology for efficient biological screening and protein synthesis in complex reaction networks, with expanded operability and less artificial interference.