Water stable nanocoatings of poly( N -isopropylacrylamide)-based block copolymers on culture insert membranes for temperature-controlled cell adhesion

作者：Masamichi Nakayama, Yuki Toyoshima, Hiroshi Chinen, Akihiko Kikuchi, Masayuki Yamato, Teruo Okano · 发表于：Journal of Materials Chemistry B · 年份：2020 · DOI：10.1039/d0tb01113d · 被引用次数：25 · 研究领域：Advanced Polymer Synthesis and Characterization、3D Printing in Biomedical Research、Polymer Surface Interaction Studies

) and the thicknesses of individual layers were relatively comparable at 10-odd nanometers. A nanothin coating scarcely inhibited the permeability of the original porous membrane. When human fibroblasts were cultured on each surface at 37 °C, the efficiencies of cell adhesion and proliferation decreased with longer PNIPAAm chains. Meanwhile, by reducing the temperature to 20 °C, longer PNIPAAm chains promoted cell detachment owing to the significant thermoresponsive alteration of cell-surface affinity. Consequently, we successfully produced a favorable cell sheet by choosing an appropriate PNIPAAm length for block copolymers.