Echinococcus granulosus (sensu stricto) (G1, G3) and E. ortleppi (G5) in Pakistan: phylogeny, genetic diversity and population structural analysis based on mitochondrial DNA

作者：Mughees Aizaz Alvi, John Asekhaen Ohiolei, Muhammad Saqib, Li Li, Muhammad Tayyab, Anum Aizaz Alvi, Yan‐Tao Wu, Bao‐Quan Fu, Hongbin Yan, Wan‐Zhong Jia · 发表于：Parasites & Vectors · 年份：2020 · DOI：10.1186/s13071-020-04199-8 · 被引用次数：42 · 研究领域：Parasitic infections in humans and animals、Amoebic Infections and Treatments、Echinoderm biology and ecology

BACKGROUND: Cystic echinococcosis (CE) is a serious tapeworm infection caused by Echinococcus granulosus (sensu lato) which infects a wide range of animals and humans worldwide. Despite the millions of livestock heads reared in Pakistan, only a few reports on CE prevalence and even fewer on the genetic diversity are available for the country. Meanwhile, the available reports on the genetic diversity are predominantly based on short sequences of the cox1 gene. METHODS: To close this knowledge gap, this study was designed to investigate the genetic diversity and population structure of Echinococcus spp. in Pakistan using the complete mitochondrial cytochrome c oxidase subunit 1 (cox1) and NADH dehydrogenase subunit 1 (nad1) genes. RESULTS: Based on BLAST searches of the generated cox1 and nad1 gene sequences from a total of 60 hydatid cysts collected from cattle (n = 40) and buffalo (n = 20), 52 isolates were identified as E. granulosus (s.s.) (G1, G3) and 8 as E. ortleppi (G5). The detection of the G5 genotype represents the first in Pakistan. The phylogeny inferred by the Bayesian method using nucleotide sequences of cox1-nad1 further confirmed their identity. The diversity indices indicated a high haplotype diversity and a low nucleotide diversity. The negative values of Tajima's D and Fu's Fs test demonstrated deviation from neutrality suggesting a recent population expansion. CONCLUSIONS: To the best of our knowledge, this report described the genetic variation of E. granu...