A plant DNA virus replicates in the salivary glands of its insect vector via recruitment of host DNA synthesis machinery

作者：Ya‐Zhou He, Yumeng Wang, Tian-Yan Yin, Elvira Fiallo‐Olivé, Yin‐Quan Liu, Linda K. Hanley-Bowdoin, Xiao‐Wei Wang · 发表于：Proceedings of the National Academy of Sciences · 年份：2020 · DOI：10.1073/pnas.1820132117 · 被引用次数：120 · 研究领域：Plant Virus Research Studies、Insect-Plant Interactions and Control、Insect symbiosis and bacterial influences

) vector and that replication is mainly in the salivary glands. We found that TYLCV induces DNA synthesis machinery, proliferating cell nuclear antigen (PCNA) and DNA polymerase δ (Polδ), to establish a replication-competent environment in whiteflies. TYLCV replication-associated protein (Rep) interacts with whitefly PCNA, which recruits DNA Polδ for virus replication. In contrast, another geminivirus, papaya leaf curl China virus (PaLCuCNV), does not replicate in the whitefly vector. PaLCuCNV does not induce DNA-synthesis machinery, and the Rep does not interact with whitefly PCNA. Our findings reveal important mechanisms by which a plant DNA virus replicates across the kingdom barrier in an insect and may help to explain the global spread of this devastating pathogen.