Human Fallopian Tube Epithelial Cell Culture Model To Study Host Responses to Chlamydia trachomatis Infection

作者：Bryan McQueen, Amy Kiatthanapaiboon, M. Leslie Fulcher, Mariam Lam, Kate Patton, Emily Powell, Avinash Kollipara, Victoria J. Madden, Robert J. Suchland, Priscilla B. Wyrick, Catherine M. O’Connell, Boris Reidel, Mehmet Kesımer, Scott H. Randell, Toni Darville, Uma M. Nagarajan · 发表于：Infection and Immunity · 年份：2020 · DOI：10.1128/iai.00105-20 · 被引用次数：28 · 研究领域：Reproductive tract infections research、Reproductive System and Pregnancy、Reproductive Physiology in Livestock

Chlamydia trachomatis infection of the human fallopian tubes can lead to damaging inflammation and scarring, ultimately resulting in infertility. To study the human cellular responses to chlamydial infection, researchers have frequently used transformed cell lines that can have limited translational relevance. We developed a primary human fallopian tube epithelial cell model based on a method previously established for culture of primary human bronchial epithelial cells. After protease digestion and physical dissociation of excised fallopian tubes, epithelial cell precursors were expanded in growth factor-containing medium.