Extraction and reconstitution of membrane proteins into lipid nanodiscs encased by zwitterionic styrene-maleic amide copolymers

作者：Mariana C. Fiori, Wan Zheng, Elizabeth Kamilar, Geuel Simiyu, Guillermo A. Altenberg, Hongjun Liang · 发表于：Scientific Reports · 年份：2020 · DOI：10.1038/s41598-020-66852-7 · 被引用次数：44 · 研究领域：Lipid Membrane Structure and Behavior、Nanopore and Nanochannel Transport Studies、RNA Interference and Gene Delivery

Membrane proteins can be reconstituted in polymer-encased nanodiscs for studies under near-physiological conditions and in the absence of detergents, but traditional styrene-maleic acid copolymers used for this purpose suffer severely from buffer incompatibilities. We have recently introduced zwitterionic styrene-maleic amide copolymers (zSMAs) to overcome this limitation. Here, we compared the extraction and reconstitution of membrane proteins into lipid nanodiscs by a series of zSMAs with different styrene:maleic amide molar ratios, chain sizes, and molecular weight distributions. These copolymers solubilize, stabilize, and support membrane proteins in nanodiscs with different efficiencies depending on both the structure of the copolymers and the membrane proteins.