Podoplanin is indispensable for cell motility and platelet-induced epithelial-to-mesenchymal transition-related gene expression in esophagus squamous carcinoma TE11A cells

作者：Nobuo Watanabe, Masako Kidokoro, Makiko Tanaka, Shigeaki Inoue, Tomoatsu Tsuji, Hisako Akatuska, Chisa Okada, Yumi Iida, Yoshinori Okada, Yusuke Suzuki, Takehito Sato, Takashi Yahata, Noriaki Hirayama, Yoshihide Nakagawa, Sadaki Inokuchi · 发表于：Cancer Cell International · 年份：2020 · DOI：10.1186/s12935-020-01328-2 · 被引用次数：23 · 研究领域：Lymphatic System and Diseases、Angiogenesis and VEGF in Cancer、Lymphatic Disorders and Treatments

BACKGROUND: The transmembrane glycoprotein podoplanin (PDPN) is upregulated in some tumors and has gained attention as a malignant tumor biomarker. PDPN molecules have platelet aggregation-stimulating domains and, are therefore, suggested to play a role in tumor-induced platelet activation, which in turn triggers epithelial-to-mesenchymal transition (EMT) and enhances the invasive and metastatic activities of tumor cells. In addition, as forced PDPN expression itself can alter the propensity of certain tumor cells in favor of EMT and enhance their invasive ability, it is also considered to be involved in the cell signaling system. Nevertheless, underlying mechanisms of PDPN in tumor cell invasive ability as well as EMT induction, especially by platelets, are still not fully understood. METHODS: Subclonal TE11A cells were isolated from the human esophageal squamous carcinoma cell line TE11 and the effects of anti-PDPN neutralizing antibody as well as PDPN gene knockout on platelet-induced EMT-related gene expression were measured. Also, the effects of PDPN deficiency on cellular invasive ability and motility were assessed. RESULTS: PDPN-null cells were able to provoke platelet aggregation, suggesting that PDPN contribution to platelet activation in these cells is marginal. Nevertheless, expression of platelet-induced EMT-related genes, including vimentin, was impaired by PDPN-neutralizing antibody as well as PDPN deficiency, while their effects on TGF-β-induced gene expression...