Effect of Quercetin on Oxidative Stress, Mitochondrial Activity, and Quality of Indian Red Jungle Fowl ( Gallus gallus murghi ) Sperm

作者：Bushra Allah Rakha, Qurrat-ul-Ain, Muhammad S. Ansari, Shamim Akhter, Ali Akhter, Muhammad Amjad Awan, J. Santiago‐Moreno · 发表于：Biopreservation and Biobanking · 年份：2020 · DOI：10.1089/bio.2020.0007 · 被引用次数：23 · 研究领域：Sperm and Testicular Function、Reproductive biology and impacts on aquatic species、Animal Genetics and Reproduction

Aim: The study was designed to elucidate the effects of quercetin in an extender on oxidative stress, mitochondrial activity and quality of Indian red jungle fowl ( Gallus gallus murghi ) sperm during cryopreservation. Materials and Methods: Semen was collected from seven adult males through abdominal massage and evaluated for semen volume, concentration, and motility. The qualifying semen ejaculates having >80% motility were diluted in red fowl extenders with 0 (control), 5, 10, 15, and 20 mM quercetin. Diluted semen was frozen following a glycerol-based protocol. Semen quality (motility, plasma membrane integrity, viability, acrosome integrity, and chromatin condensation status) and biochemical parameters (mitochondrial activity, ferric reducing antioxidant power, and malondialdehyde [MDA]) were determined at various stages of cryopreservation. Results: Sperm motility, plasma membrane integrity, viability, acrosome integrity, and chromatin condensation were recorded highest ( p < 0.05) with 15 mM quercetin compared with 5, 10, and 20 mM quercetin and control at post-dilution, cooling, equilibration, and freeze–thawing. Nevertheless, mitochondrial activity and antioxidant potential were recorded highest with 15 mM quercetin compared with all experimental extenders at post-equilibration and freeze–thawing. MDA concentration in sperm and seminal plasma were recorded lowest ( p < 0.05) in the extender having 15 mM quercetin at post-equilibration and freeze–thawing. Cry...