Function analysis and stress-mediated cis -element identification in the promoter region of VqMYB15

作者：Ruixiang Li, Fanding Zhu, Dong Duan · 发表于：Plant Signaling & Behavior · 年份：2020 · DOI：10.1080/15592324.2020.1773664 · 被引用次数：93 · 研究领域：Horticultural and Viticultural Research、Plant biochemistry and biosynthesis、Plant Gene Expression Analysis

The transcription factor MYB15 plays an important role in grape basal immunity, and its promoter can be used as a potential target in resistance breeding. However, the regulatory mechanisms of cis-elements in its promoter region under a variety of stresses remain unclear. In this study, we identified some putative cis-regulatory elements present upstream of MYB15 in Vitis quinquangularis Shanyang (pVqMYB15_SY) and subsequently characterized the function of these elements using reporter assays. Our results showed that TCA-elements 1 and 2, ABRE, MYC and 3-AF1 binding site 1 are key cis-regulatory elements in pVqMYB15_SY and play important roles in plant bio/abiotic stress resistance.