Insights of Molecular Mechanism of Xylem Development in Five Black Poplar Cultivars

作者：Lei Zhang, Bobin Liu, Jin Zhang, Jianjun Hu · 发表于：Frontiers in Plant Science · 年份：2020 · DOI：10.3389/fpls.2020.00620 · 被引用次数：23 · 研究领域：Bioenergy crop production and management、Plant Gene Expression Analysis、Plant Molecular Biology Research

Black poplar (Populus deltoides, P. nigra and their hybrids) is the main poplar cultivars in China, it offers interesting options large-scale biomass production for bioenergy due to its rapid growth and high yield. Poplar wood properties were associated with chemical components and physical structures during wood formation. In this study, five poplar cultivars, P. euramericana ‘Zhonglin46’ (Pe1), P. euramericana ‘Guariento’ (Pe2), P. nigra ‘N179’ (Pn1), P. deltoides ‘Danhong’ (Pd1), and P. deltoides ‘Nanyang’ (Pd2), were used to explore the molecular mechanism of xylem development. We analyzed the structural differences of developing xylem in the five cultivars and profiled the transcriptome-wide gene expression patterns through RNA-Sequencing. The cross sections of the developing xylem showed that the cell wall thickness of developed fiber in Pd1 was thickest and the number of xylem vessels of Pn1 was the least. A total of 10,331 differentially expressed genes were identified among ten pairwise comparisons of the five cultivars, most of them were related to programmed cell death and secondary cell wall thickening. K-means cluster analysis and gene ontology enrichment analysis showed that the genes highly expressed in Pd1 were related to nucleotide decomposition, metabolic process, transferase and microtubule cytoskeleton; whereas the genes highly expressed in Pn1 were involved in cell wall macromolecule decomposition and polysaccharide binding processes. Based on a weighted ...