Polymer-Based Bioorthogonal Nanocatalysts for the Treatment of Bacterial Biofilms

作者：Rui Huang, Cheng‐Hsuan Li, Roberto Cao‐Milán, Luke D. He, Jessa Marie Makabenta, Xianzhi Zhang, Erlei Yu, Vincent M. Rotello · 发表于：Journal of the American Chemical Society · 年份：2020 · DOI：10.1021/jacs.0c01758 · 被引用次数：142 · 研究领域：Click Chemistry and Applications、Advanced biosensing and bioanalysis techniques、Nanoplatforms for cancer theranostics

Bioorthogonal catalysis offers a unique strategy to modulate biological processes through the in situ generation of therapeutic agents. However, the direct application of bioorthogonal transition metal catalysts (TMCs) in complex media poses numerous challenges due to issues of limited biocompatibility, poor water solubility, and catalyst deactivation in biological environments. We report here the creation of catalytic "polyzymes", comprised of self-assembled polymer nanoparticles engineered to encapsulate lipophilic TMCs. The incorporation of catalysts into these nanoparticle scaffolds creates water-soluble constructs that provide a protective environment for the catalyst. The potential therapeutic utility of these nanozymes was demonstrated through antimicrobial studies in which a cationic nanozyme was able to penetrate into biofilms and eradicate embedded bacteria through the bioorthogonal activation of a pro-antibiotic.