Expression of 14-3-3ε in bladder urothelial carcinoma and its correlation with clinicopathologic features

作者：Yuanyuan Zhao, Yanfeng Xi, Xuanqin Yang, Shuxiong Tian, Nan Lyu · 发表于：肿瘤研究与临床 · 年份：2014 · DOI：10.3760/cma.j.issn.1006-9801.2014.10.006 · 研究领域：14-3-3 protein interactions、Microbial metabolism and enzyme function、Ubiquitin and proteasome pathways

Objective To investigate the expression of 14-3-3e protein in the bladder urothelial carcinoma (BUC) and to explore its association with the clinicopathologic features.Methods The bladder urothelial carcinoma samples were divided into three groups:normal control group of 10 cases,low-grade malignant BUC group of 25cases (includes 5 cases of papilloma,10 cases of PUNLMP and 10 cases of low grade non-invasive papillary urothelial carcinoma),high-grade malignant BUC group of 21 cases (includes 11 cases of high-grade non invasive papillary urothelial carcinoma and 10cases of infiltrating carcinoma).The expression and location of 14-3-3e in three groups were detected by immunohistochemical EnVision and the relationship with clinicopathologic parameters was analyzed.Results 14-3-3e expression was observed in the cytoplasm of the cell.The expression of 14-3-3e in normal control group was 90 ％ (9/10),low-grade malignant BUC group was 72.0 ％ (18/25),high grade malignant BUC group was 14.3 ％ (3/21).It correlated with histological grading but had not showed correlation with other clinicopathologic parameters.There was significant difference in 14-3-3e expression between the high grade malignant BUC group and the low-grade malignant BUC group,the high grade malignant BUC group and norml control group (all P ＜ 0.05).Conclusions 14-3-3e plays an important role in carcinogenesis of BUC.It may be a biomarker for early diagnosis and classification of BUC and shows promise for clinic applicati...