An association between single nucleotide polymorphisms of IL-23R gene and Keshan disease in Northwest Chinese Han population

作者：Peiru Du, Wei Wu, Xuerong Hou, Yingting Wang, Jie Zhang, Zuogong Liu, Wuhong Tan · 发表于：Chin J Endemiol · 年份：2019 · DOI：10.3760/cma.j.issn.2095-4255.2019.05.004 · 研究领域：Whipple's Disease and Interleukins、Toxin Mechanisms and Immunotoxins、Cytokine Signaling Pathways and Interactions

Objective To investigate the relationship between single nucleotide polymorphisms of interleukin 23 receptor (IL-23R) gene and Keshan disease (KD) in Northwest Chinese Han population. Methods A total of 285 Chinese Han subjects from Huangling, Shaanxi, including 79 KD patients (case group) and 206 control subjects (control group) were involved in this study. Genomic DNA was extracted from peripheral venous blood. The polymorphism of genetic variation was genotyped by matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF). All sample groups were tested for Hardy-Weinberg equilibrium using goodness-of-fit χ2 test. Differences in genotype distribution between two groups were compared by χ2 test. Logistic regression analysis was applied to detect association using age as a confounding factor. Results The gene frequency distribution of IL-23R gene rs10889677 in case group and control group conformed to the Hardy-Weinberg equilibrium (χ2=0.254, P > 0.05). Correlation analysis results: the difference of genotype frequency of IL-23R gene rs10889677 in case group (CC, CA, AA were 6.3%, 36.7%, 57.0%, respectively) and control group (CC, CA, AA were 5.3%, 43.2%, 51.5%, respectively) was not statistically significant (χ2=1.008, P > 0.05). After age adjustment, there was no significant difference in genotype frequency of IL-23R gene rs10889677(χ2adj=0.669,P>0.05)between two groups. Conclusion There is no correlation between IL-23R gene rs10889677 and KD ...