Wogonin Suppresses IL‐10 Production in B Cells via STAT3 and ERK Signaling Pathway

作者：Fan Li, Dongbo Qiu, Guo N. Huang, Jingrou Chen, Qiongli Wu, Shiqiu Xiong, Changyou Wu, Yanwen Peng, Qi Zhang · 发表于：Journal of Immunology Research · 年份：2020 · DOI：10.1155/2020/3032425 · 被引用次数：19 · 研究领域：Flavonoids in Medical Research、Natural product bioactivities and synthesis、NF-κB Signaling Pathways

Wogonin (5,7‐dihydroxy‐8‐methoxyflavone) is an ingredient of the extracts from Scutellaria baicalensis, which has documented a wide spectrum of anti‐inflammatory and antitumor activities, including inhibiting regulatory T cells, regulating effector T cell functions, and mediating macrophage immunity. However, the potential effect of Wogonin on B cells has not been fully understood. Here, our results showed that Wogonin inhibited IL‐10 secretion in B cells. When purified B cells were activated by lipopolysaccharide (LPS) in vitro, the amount of IL‐10 production in supernatant was decreased by Wogonin significantly. The protective role of B cells on dextran sulfate sodium‐ (DSS‐) induced colitis was alleviated after exposure to Wogonin. Furthermore, administration of Wogonin on LPS‐treated B cells suppressed phosphorylation of STAT3 and ERK, but not AKT. Interestingly, among those IL‐10 signaling‐associated transcription factors, mRNA and protein levels of Hif‐1 α were specifically decreased by Wogonin. Overall, our study indicates that Wogonin suppresses potentially IL‐10 production in B cells via inhibition of the STAT3 and ERK signaling pathway as well as inhibition of mRNA and protein levels of the transcription factor Hif‐1 α . These results provide novel and potential molecular targets of Wogonin in B cells and help us further understand its mechanism of action, which could potentially improve its clinical application in the future.