Effect of penhyclidine hydrochloride on blood-brain barrier permeability in a rat model of lipopolysaccharide-induced brain injury

作者：Peng Yang, Daowen Si, Yun Li, Nan Liu, Ying Wu, Yunhe Zhang, Limin Zhang · 发表于：Zhonghua chuangshang zazhi · 年份：2015 · DOI：10.3760/cma.j.issn.1001-8050.2015.11.014 · 研究领域：Traditional Chinese Medicine Analysis、Neurological Disease Mechanisms and Treatments、Immune Response and Inflammation

Objective To detect the effect of penhyclidine hydrochloride (PHC) on blood-brain barrier permeability in rats with lipopolysaccharide (LPS)-induced brain injury. Methods Eighty-one clear adult male SD rats were divided into control group, LPS group and PHC group according to the random number table, with 27 rats per group. The model of LPS-induced brain injury was established both in LPS and PHC groups. Rats were treated intraperitoneally with 2 mg/kg PHC in PHC group, while with the same volume of normal saline in control and LPS groups. Evans blue propagation through the brain was detected under the fluorescence microscope. Evans blue content was measured with a spectrophotometer. TNF-α content was measured with the Elisa assay. Brain water content was assessed with wet-to-dry weight ratios. Results Evans blue distribution in the cortex was obvious in LPS group, but decreased in PHC group. In control group contents of Evans blue in the cortex [(10.2±1.4)μg/g] and in the hippocampus[(11.3±0.8)μg/g] lowered as compared to these in LPS group [(234.8±14.7) and (23.1±1.9)μg/g] and PHC group [(71.0±8.7) and (15.2±1.9)μg/g] (P<0.01). Brain water content in control group was (73.5±1.8)%, increased to (85.3±1.6)% in LPS group and (78.9±1.2)% in PHC group (P<0.01). In control group contents of TNF-α in blood serum [(40.6±4.4) pg/ml], cerebrospinal fluid [(84.0±8.6)pg/ml] and brain tissue [(156.3±11.8) pg/ml] were significantly lower than these in LPS group[(93.4±7.2), (...