The role of extracellular vesicles from stored RBC units in B lymphocyte survival and plasma cell differentiation

作者：Yuhan Gao, Haiqiang Jin, Hui Tan, Yan Wang, Jia Wu, Yuqing Wang, Jianhua Zhang, Ying Yang, Wenqin Tian, Ruiqin Hou · 发表于：Journal of Leukocyte Biology · 年份：2020 · DOI：10.1002/jlb.1a0220-666r · 被引用次数：16 · 研究领域：Extracellular vesicles in disease、Erythrocyte Function and Pathophysiology、Thermal Regulation in Medicine

Extracellular vesicles (EVs) are small, double-membrane vesicles derived from erythrocytes, leukocytes, platelets, and cells of multiple tissues under physiologic or pathologic conditions. The role of EVs in stored RBC units is of great interest with respect to transfusion-related immunomodulation. The current study focuses on the quantity of EVs isolated from stored RBC units and their action on B cell-mediated immune responses. The in vitro experiment demonstrated that EVs exhibited a negative role in B cell survival, plasmacytic differentiation, and class switch recombination under LPS stimulation. Furthermore, LPS-induced antibody production was significantly decreased after EVs injection in vivo. Biochemical analysis revealed that EVs hampered the expression of Blimp-1 and IRF4 and the activation of NF-κB pathway in LPS-primed B cells. Overall, these data imply a vital role for EVs isolated from RBC units in B cell-mediated immune responses.