Purification of Protein-complexes from the Cyanobacterium Synechocystis sp. PCC 6803 Using FLAG-affinity Chromatography

作者：Minna M. Koskela, Petra Skotnicová, Éva Kiss, Roman Sobotka · 发表于：BIO-PROTOCOL · 年份：2020 · DOI：10.21769/bioprotoc.3616 · 被引用次数：16 · 研究领域：Photosynthetic Processes and Mechanisms、Algal biology and biofuel production、Protist diversity and phylogeny

. Soluble proteins or solubilized thylakoids are subjected to a single affinity purification step that utilizes the highly specific binding of FLAG-affinity resin. After an intensive wash, the captured proteins are released from the resin under native conditions using an excess of synthetic 3x FLAG peptide. The protocol allows fast isolation of low abundant protein complexes with a superb purity.