[rotective effect of bone marrow mesenchymal stem cells-derived exosomes against testicular ischemia-reperfusion injury in rats].

作者：Wansong Zhang, Cheng Yang, Wenbin Guo, Xiaobin Guo, Jun Bian, Qi-Zhao Zhou, Mingkun Chen, Junhao Zhou, Zijian Chen, Peng Wang, Xian-yuan Lv, Zhuoyu Xiao, Cundong Liu · 发表于：PubMed · 年份：2018 · DOI：10.3969/j.issn.1673-4254.2018.08.02 · 被引用次数：12 · 研究领域：Extracellular vesicles in disease、Testicular diseases and treatments、Sperm and Testicular Function

OBJECTIVE: To investigate the protective effect of bone marrow mesenchymal stem cells (BMSCs)-derived exosomesagainst testicular ischemia-reperfusion injury (IRI) in rats. METHODS: Rat BMSCs were isolated, cultured and identified in theprimary culture. The exosomes were extracted from the BMSCs and characterized using nanoparticle tracking analysis, transmission electron microscopy, and Western blotting. Twenty-four healthy male SD rats were randomly divided into shamoperation group, testicular IRI with saline treatment group and IRI with exosome treatment group. The contralateral testes ofthe rats were collected for pathological observation, aseessment of superoxide dismutase (SOD) and malondialdehyde (MDA), and detection of HMGB1, caspases-3 and cleaved caspase-3 expressions using Western blotting. RESULTS: < 0.05). CONCLUSIONS: BMSCs-derived exosomes protects against testicular IRI due to the anti-oxidant, antiinflammatory and anti-apoptosis activities of the exosomes.