Quercetin alleviated H 2 O 2 ‐induced apoptosis and steroidogenic impairment in goat luteinized granulosa cells

作者：Manman Li, Xue Yang, Hao Yu, Dagan Mao · 发表于：Journal of Biochemical and Molecular Toxicology · 年份：2020 · DOI：10.1002/jbt.22527 · 被引用次数：13 · 研究领域：Reproductive Biology and Fertility、Reproductive Physiology in Livestock、Genomics, phytochemicals, and oxidative stress

Abstract Quercetin (Que) is a natural flavonoid in most plants. Luteinized granulosa cell (LGC) culture is necessary for the study of follicle growth/differentiation. In the present study, we analyzed the role of Que in steroid production and apoptosis in hydrogen peroxide (H 2 O 2 )‐treated goat LGCs. The results showed that treatment with H 2 O 2 induced apoptosis in goat LGCs, and treatment with Que decreased LGC apoptosis induced by H 2 O 2 ( P < .05), accompanied with the different expressions of BAX, BCL‐2, Caspase 3 , and Cleaved caspase 3 . Meanwhile, the messenger RNA expressions of nuclear factor erythroid 2 like 2 ( Nrf2 ) and its downstream genes were upregulated with H 2 O 2 +Que treatment, accompanied by the increased cellular viability ( P < .05). Furthermore, Que alleviated H 2 O 2 ‐induced reduction in the secretion of progesterone (P 4 ) ( P < .05); however, it had no effect on the secretion of estrogen (E 2 ). Simultaneously, the expressions of StAR and P450scc were increased when treated with Que +H 2 O 2 , compared with the group treated with only H 2 O 2 ( P < .05). In conclusion, it is observed that Que could alleviate the H 2 O 2 ‐induced apoptosis and steroidogenic impairment in goat LGCs, which might be mediated by the Nrf2 pathway.