GAS CHROMATOGRAPHY OF BACTERIAL WHOLE CELL METHANOLYSATES.

作者：Erik Jantzen, Klaus Bryn, Kjell Bøvre · 发表于：Acta Pathologica Microbiologica Scandinavica Section B Microbiology and Immunology · 年份：1974 · DOI：10.1111/j.1699-0463.1974.tb02373.x · 被引用次数：21 · 研究领域：Analytical Chemistry and Chromatography、Microbial Metabolic Engineering and Bioproduction、Metabolomics and Mass Spectrometry Studies

A procedure for determination of the fatty acid and monosaccharide composition of bacterial whole cell preparations has been developed. Specific elution profiles of total fatty acids, hydroxy fatty acids and monosaccharides from 1–10 mg dry cells are obtained. The method is based on the following five steps: 1. Selective depolymerization of washed, lyophilized whole cells by treatment with HCl in anhydrous methanol. 2. Trifluoroacetylation of released and solubilized monomeric compounds. 3. Fractionation of the trifluoroacetylated methanolysate by an acetonitrile/hexane two phase solvent system. 4. Analyses of the fractions by gas‐liquid chromatography. 5. Peak identification by retention characteristics, selective extraction, reduction, and by mass spectrometry.