[Simultaneous determination of 12 monohydroxylated polycyclic aromatic hydrocarbon metabolites in human urine by ultra-performance liquid chromatography coupled with triple quadrupole mass spectrometry].

作者：Hui Fu, Xiaojian Hu, Xi Chen, Lin Shaobin · 发表于：PubMed · 年份：2018 · DOI：10.3724/sp.j.1123.2017.12029 · 被引用次数：1 · 研究领域：Metabolomics and Mass Spectrometry Studies、Analytical Chemistry and Chromatography

HSS T3 column (100 mm×2.1 mm, 1.8 μm) with a mobile phase comprising methanol and water. The analytes were detected by electrospray ionization (ESI)-MS/MS in negative mode and multiple reaction monitoring (MRM) mode. The linear ranges of the 12 metabolites were 0.04-20.0 μg/L with correlation coefficients above 0.99. The detection limits were in the range of 0.01-0.41 μg/L and the recoveries were 80.0%-105%. The intra-group relative standard deviations (RSDs) were less than 9.12% and the inter-group RSDs were less than 19.7%. This method was applied to the analysis of 100 urine samples and the results showed that the method was simple, sensitive and selective.